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Evaluation of nonstarch polysaccharides and oligosaccharide content of different soybean varieties (Glycine max) by near-infrared spectroscopy and proteomics.

Hollung K, Øverland M, Hrustić M, Sekulić P, Miladinović J, Martens H, Narum B, Sahlstrøm S, Sørensen M, Storebakken T, Skrede A

Matforsk, The Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. kristin.hollung@matforsk.no

A total of 832 samples of soybeans were screened by near-infrared (NIR) reflectance spectroscopy, to identify soybean samples with a lower content of oligosaccharides and nonstarch polysaccharides (NSP). Of these, 38 samples were identified on the basis of variation in protein content and agronomic value and submitted to high-resolution NIR spectroscopy. On the basis of the NIR data, 12 samples were further selected for chromatographic characterization of carbohydrate composition (mono-, di-, and oligosaccharides and NSP). Their soluble proteins were separated by two-dimensional gel electrophoresis (2DE). Using partial least-squares regression (PLSR), it was possible to predict the content of total NSP from the high-resolution NIR spectra, suggesting that NIR is a suitable and rapid nondestructive method to determine carbohydrate composition in soybeans. The 2DE analyses showed varying intensities of several proteins, including the glycinin G1 precursor. PLSR analysis showed a negative correlation between this protein and insoluble NSP and total uronic acid (UA).

Published 9 November 2005 in J Agric Food Chem, 53(23): 9112-21.
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