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Real-time polymerase chain reaction quantification of the transgenes for roundup ready corn and roundup ready soybean in soil samples.

Lerat S, England LS, Vincent ML, Pauls KP, Swanton CJ, Klironomos JN, Trevors JT

Department of Environmental Biology, of Plant Agriculture, and of Botany, University of Guelph, Guelph, Ontario, Canada.

A method for quantification of recombinant DNA for Roundup Ready (RR) corn and RR soybean in soil samples is described. Soil DNA from experimental field samples was extracted using a soil DNA extraction kit with a modified protocol. For the detection and quantification of recombinant DNA of RR corn and RR soybean, a molecular beacon and two pairs of specific primers were designed to differentially target recombinant DNA in these two genetically modified crops. Soil DNA extracts were spiked with RR corn or RR soybean DNA, and recombinant DNA was quantified using real-time PCR with a molecular beacon. As few as one copy of RR corn genome or one copy of RR soybean genome was detected in the soil DNA extract.

Published 2 March 2005 in J Agric Food Chem, 53(5): 1337-42.
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