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Event-specific real-time detection and quantification of genetically modified Roundup Ready soybean.

Huang CC, Pan TM

Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan 106.

The event-specific real-time detection and quantification of Roundup Ready soybean (RRS) using an ABI PRISM 7700 sequence detection system with light upon extension (LUX) primer was developed in this study. The event-specific primers were designed, targeting the junction of the RRS 5' integration site and the endogenous gene lectin1. Then, a standard reference plasmid was constructed that carried both of the targeted sequences for quantitative analysis. The detection limit of the LUX real-time PCR system was 0.05 ng of 100% RRS genomic DNA, which was equal to 20.5 copies. The range of quantification was from 0.1 to 100%. The sensitivity and range of quantification successfully met the requirement of the labeling rules in the European Union and Taiwan.

Published 11 May 2005 in J Agric Food Chem, 53(10): 3833-9.
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