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Analysis of glyphosate and aminomethylphosphonic acid by capillary electrophoresis with electrochemiluminescence detection.

Chiu HY, Lin ZY, Tu HL, Whang CW

Department of Chemistry, Tunghai University, Taichung 407, Taiwan.

A capillary electrophoresis (CE) method coupled with electrochemiluminescence (ECL) detection for the analysis of glyphosate (GLY) and its major metabolite aminomethylphosphonic acid (AMPA) is presented. Complete separation of GLY and AMPA was achieved in 8min using a background electrolyte of 20mM sodium phosphate (pH 9.0) and a separation voltage of 21kV. ECL detection was performed with an indium tin oxide (ITO) working electrode bias at 1.6V (vs. a Pt-wire reference) in a 300mM sodium phosphate buffer (pH 8.0) containing 3.5mM Ru(bpy)(3)(2+) (where bpy=2.2'-bipyridyl). Linear correlation (r>/=0.997) between ECL intensity and analyte concentration was obtained in the ranges 0.169-16.9 and 5.55-111mugml(-1) for GLY and AMPA, respectively. The limits of detection (LODs) for GLY and AMPA in water were 0.06mugml(-1) and 4.04mugml(-1), respectively. The developed method was applied to the analysis of GLY in soybeans. The LOD of GLY in soybean was 0.6mugg(-1). Total analysis time including sample pretreatment was less than 1h.

Published 21 December 2007 in J Chromatogr A, 1177(1): 195-8.
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