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Evaluation of alpha-galactosidase biosynthesis by Streptomyces griseoloalbus in solid-state fermentation using response surface methodology.

Anisha GS, Sukumaran RK, Prema P

Biotechnology Division, National Institute for Interdisciplinary Science and Technology (Formerly Regional Research Laboratory), CSIR, Trivandrum, Kerala, India.

AIM: The present study was aimed at evaluating the effects of the three crucial factors, galactose concentration, inoculum size and moisture content, on alpha-galactosidase production by the filamentous actinobacterium Streptomyces griseoloalbus in solid-state fermentation. METHODS AND RESULTS: Central Composite design was adopted to derive a statistical model for the optimization of fermentation conditions. Maximum alpha-galactosidase yield (117 U g(-1) of dry fermented substrate) was obtained when soya bean flour supplemented with 1.5% galactose and with initial moisture content of 40% was inoculated with 1.9 x 10(6) CFU g(-1) initial dry substrate. CONCLUSIONS: The model was valid and could result in considerably enhanced enzyme yield. SIGNIFICANCE AND IMPACT OF THE STUDY: The results indicated a cost effective method for the production of alpha-galactosidase using soya bean flour. This is the first report on exploitation of the potential of filamentous bacterium for the production of alpha-galactosidase, an enzyme having versatile applications.

Published 22 February 2008 in Lett Appl Microbiol, 46(3): 338-43.
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